Synergistic Hypolipidemic Effects of Ascorbic Acid and Creatine Supplementation in Male Rabbits: A Biochemical and Statistical Analysis
DOI:
https://doi.org/10.65336/WJAMS.2026.3501Keywords:
Ascorbic Acid, Creatine, Lipid profile, RabbitsAbstract
Background: Dyslipidemia is a critical risk factor for cardiovascular diseases and metabolic syndromes, necessitating the exploration of safe and effective lipid-lowering interventions. Ascorbic acid (vitamin C), a potent antioxidant, has been associated with improved lipid metabolism, while creatine (CrS), known for its role in cellular energy homeostasis, may also influence lipid pathways. The combined impact of these two supplements on lipid profiles, however, remains underexplored in animal models. Aim: This study aimed to investigate the individual and combined effects of ascorbic acid and creatine supplementation on plasma lipid profile markers triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) in male rabbits. Methods: Twenty male rabbits were randomly divided into four groups (n = 5 per group): control, ascorbic acid, creatine (CrS), and ascorbic acid + CrS combination. The animals were treated for a defined experimental period. Blood samples were collected and analyzed for TG, TC, LDL-c, and HDL-c levels. One-way ANOVA was used for statistical analysis to evaluate group differences, with significance set at p < 0.05. Results were expressed as mean ± SE, and Tukey's post hoc test was applied to compare group means. Results: Triglycerides (TG): All treatment groups showed significant reductions in TG levels compared to control (F = 15.06, p = 0.000), with the most significant decrease observed in the ascorbic acid + CrS group (42.60 ± 1.718 mg/dL). Total Cholesterol (TC): A significant reduction in TC was observed in the combination group (77.65 ± 14.534 mg/dL) compared to the control group (109.60 ± 4.216 mg/dL) (F = 11.42, p = 0.000). LDL-c: LDL-c levels were significantly reduced in both the CrS (53.95 ± 2.233 mg/dL) and the combination group (52.72 ± 1.479 mg/dL), compared to the control (F = 15.10, p = 0.000). HDL-c: The highest HDL-c levels were recorded in the ascorbic acid group (49.05 ± 1.439 mg/dL), followed by the combination group (50.63 ± 1.715 mg/dL), both significantly higher than control (F = 3.25, p = 0.024). Conclusion: The combined administration of ascorbic acid and creatine produced the most significant improvements in lipid profiles, suggesting a potential synergistic effect in reducing cardiovascular risk factors. These findings support the therapeutic potential of combined antioxidant and energy-modulating supplementation in lipid regulation, paving the way for future translational research in metabolic syndrome and cardiovascular prevention strategies.
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