Chemical Profiling of Fenugreek (Trigonella foenum-graecum L.) Seeds and Their Hematological Modulatory Effects in Adult Male Rabbits: A Dose-Response Study
Keywords:
Fenugreek, Trigonella foenum-graecum, phytochemicals, hematology, rabbitsAbstract
This study investigated the phytochemical and nutritional composition of Trigonella foenum-graecum (fenugreek) seeds and evaluated their effects on hematological parameters in male rabbits. The phytochemical analysis revealed appreciable levels of bioactive compounds, including total saponins (6.25 ± 0.42%), diosgenin (1.47 ± 0.08%), total flavonoids (37.84 ± 2.16 mg QE/g), total polyphenols (76.92 ± 4.35 mg GAE/g), and trigonelline (0.31 ± 0.02%). In addition, the seeds exhibited a high crude protein content (27.85 ± 1.12%) with moderate levels of fiber, moisture, and ash. Hematological evaluation showed that fenugreek supplementation significantly improved red blood cell count, hemoglobin concentration, and packed cell volume in treated groups compared with the control group, with dose-dependent variations. Mean corpuscular indices (MCV, MCH, and MCHC) remained relatively stable across all groups. Overall, fenugreek seed supplementation demonstrated a positive influence on hematological status in male rabbits, indicating its potential nutritional and physiological benefits.
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