The Protective Role of Alpha-Lipoic Acid against Paracetamol-Induced Hematotoxicity in Male Rabbits
Keywords:
Paracetamol, Alpha-Lipoic Acid, Antioxidants, Male Rabbits, Oxidative StressAbstract
Background: Paracetamol (PAR) is a widely used analgesic that can cause hematological toxicity at high doses through oxidative stress. Alpha-lipoic acid (ALA) is a potent antioxidant known for its cytoprotective properties. Objective: This study aimed to evaluate the protective effects of ALA against PAR-induced hematological alterations in male rabbits. Methods: Male rabbits were divided into four groups: Control, PAR-treated, ALA-treated, and a combination group (PAR+ALA). Hematological parameters, including Red Blood Cells (RBCs), White Blood Cells (WBCs), Hemoglobin (Hb), Hematocrit (HCT), and Platelets (PLAT), were analyzed. Results: The administration of PAR led to a significant decrease in RBC count (5.36 ±0.111 ×106µL) and WBC count (6.9 ±0.37 ×106µL), suggesting myelosuppression or oxidative damage. In contrast, ALA supplementation markedly improved the erythropoietic profile, significantly increasing RBCs ( 6.79 ±0.258 ×106µL) and HCT (45.04 ±0.783%). Interestingly, the PAR+ALA combination group showed a significant recovery in RBC and WBC levels compared to the PAR-only group, indicating a clear protective role for ALA. While Hb and Platelet levels showed no statistically significant differences across groups (p>0.05), a declining trend in platelets was observed in the PAR group, which was stabilized by ALA co-treatment. Conclusion: The findings demonstrate that alpha-lipoic acid effectively mitigates the hematotoxic effects of paracetamol in male rabbits. This protection is likely due to ALA’s antioxidant capacity, which preserves red cell membrane integrity and supports bone marrow function.
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